Chromatographic Studies of Nucleic Acids 1. A TECHNIQUE FOR THE IDENTIFICATION AND ESTIMATION OF PURINE AND PYRIMIDINE BASES, NUCLEOSIDES AND RELATED SUBSTANCES BY R. MARKHAM AND J. D. SMITH

Fig. 8. Electrophoretic patterns of oxyhaemoglobins. All experiments, with the exception of nos. 13-15, in phosphate, pH 8; I, 0*05; 14 V./cm. Nos. 1 and 2 ascending and descending patterns of rabbit HbO2, concentration = 1-35 g./100 ml., after 430 and 435 min. Nos. 3-15 patterns of mixtures 1:1 of hubman and rabbit HbO2. Left, ascending limb; right, descending limb. Nos. 3 and 4 =Exp. 1 ofTable 4,after 320 and322min.;no. 5 =Exp. 2, after 299 min.; nos. 6 and 7 =Exp. 3, after 321 and 209 min.; nos. 8 and 9 =Exp. 6, after 382 and 385 min.; nos. 10-12 =Exp. 7, after 243, 360and364min.; nos. 13and 14 ascending patterns, no. 15 descending pattern of a mixture 1:1 of human and rabbit HbO2 in phosphate pH 8, !u=0-2; nos. 13 and 15 after 443 min. at 7V./cm., then 160min. at 6V./cm.; no. 14after443min. at 7V./cm., then 173 min. at 6V./cm., then 815 min. at 4V./cm. Total distance migrated about 9 cm.